| Depart 100 University Ave, Toronto ON on Hwy-11A [University Ave] (South) for 0.5 mi |
| Take Ramp (RIGHT) onto Hwy-2 [Frederick G Gardiner Expy] for 5.2 mi towards Gardiner Expwy West / Qew |
| At exit 145, road name changes to Frederick G Gardiner Expy [Gardiner Expy] for 4.2 mi |
| Road name changes to Qew [Queen Elizabeth Way] for 10.3 mi |
| Merge onto Hwy-403 [Qew] for 13.1 mi |
| Keep LEFT onto Qew [Queen Elizabeth Way] for 3.0 mi towards Q.E.W. / Niagara / Fort Erie |
| Road name changes to James N Allen Skyway Bridge [Qew] for 2.9 mi |
| Road name changes to Qew [Queen Elizabeth Way] for 30.1 mi |
| Road name changes to Garden City Skyway [Qew] for 1.9 mi |
| Road name changes to Qew [Queen Elizabeth Way] for 1.5 mi |
| At exit 37, keep LEFT onto Ramp for 0.3 mi towards Hwy-405 / Queenston |
| Road name changes to Hwy-405 for 5.2 mi |
| Road name changes to Queenston-Lewiston Bridge for 0.5 mi |
| Entering New York |
| Road name changes to I-190 [Niagara Expy] for 14.8 mi |
| At exit 16, take Ramp (RIGHT) onto I-290 [Youngmann Expy] for 9.8 mi towards I-290 / I-90 / Tonawanda / Rochester |
| Turn LEFT onto Ramp for 0.6 mi towards I-90 / Albany |
| *Toll road* Merge onto I-90 [Gov Thomas E Dewey Thruway] for 92.5 mi |
| At exit 42, turn RIGHT onto Ramp for 0.5 mi towards RT-14 / Geneva / Lyons |
| Take Ramp (RIGHT) onto SR-14 for 0.4 mi |
| Take Ramp (RIGHT) onto SR-96 for 6.6 mi towards RT-96 / Waterloo |
| Turn RIGHT to stay on SR-96 for 1.2 mi |
| Turn RIGHT (West) onto SR-96 [CR-117] for 98 yds |
| Turn LEFT (South) onto SR-96 for 9.0 mi |
| Stay on SR-96 (South) for 4.2 mi |
| Bear RIGHT (South) onto SR-414 [SR-96] for 2.5 mi |
| Turn LEFT (East) onto SR-96 [N Town Line Rd] for 24.9 mi |
| Keep STRAIGHT onto SR-89 [SR-96] for 164 yds |
| Turn RIGHT (South) onto SR-13 [SR-34] for 0.2 mi |
| Turn LEFT (East) onto SR-79 [W Green St] for 1.3 mi |
| Turn LEFT (East) onto SR-366 [Mitchell St] for 0.2 mi |
| Bear LEFT (North-East) onto SR-366 [Ithaca Rd] for 0.3 mi |
| Keep STRAIGHT onto SR-366 [Dryden Rd] for 0.5 mi |
| At this 4 way stop turn left into the Wilson Lab Parking lot |
