| NON-Hazardous: | |
| Form: | Already incorporated in crystals, the crystals being mounted in capillaries, frozen, or contained in small hanging drops, wells or vials in solution in small hanging drops, wells, or vials. "Small" means less than 1ml. |
| Total Quantity: | No more than 1/50 the LD50 for a 50kg animal or 0.01 millimoles if the LD50 is not available. LD-50's, when known, are given on Material Safety Data Sheets. |
| Manipulations: | Limited to transfer of crystals in and out of solutions, and transfer of small amounts of solution from one container to another. |
| Hazardous: | You must supply CHESS with a Heavy Atom Compounds Declaration, listing the type and amount of each compound. |
| Form: | In solution or dry in closed containers. |
| Total Quantity: | Between 1/50 and 1/2 the LD50 for a 50kg animal, or between 0.01 and 0.25 millimoles if the LD50 is not available. |
| Manipulations: | Mixing / dilution of solutions, transfer of crystals into and out of solutions. |
| Unacceptable at CHESS - Quantities greater than those given in the "hazardous" category. | |
| All crystals, solutions, and dry samples containing heavy atom
compounds should be transported into and out of CHESS in
double-sealed containers.
In the case of non-hazardous experiments, spilled samples may be cleaned up with tissues or paper towels and the affected area washed with soap and water. In the unlikely case of spillage of multiple samples, notification of the CHESS operator to supervise cleanup is recommended. In the case of hazardous experiments, heavy atom solutions are to be kept in a designated location, which will normally be Room 172 Wilson for A1 or F2 users, and the prep area of the BL-3 facility for F1 users. The work area in this location will be clearly demarcated by the experimenters, and their solutions and affected glassware and equipment should not be used by anyone else. It is recommended to cover the workbench in this area with absorbent material (e.g. paper towels) to absorb spills. For larger volumes of solution, pans on the bench top to contain spills are appropriate. Small spills may be cleaned up with tissues or paper towels and soap and water, as for the non-hazardous situation. In the event of larger spills, notification of the CHESS operator to supervise cleanup is required. Crystals containing heavy atoms, and small amounts of heavy atom solution, may then be transported to the experimental stations, preferably in covered containers. Dry heavy atom compounds should remain sealed until dissolved, and be opened only in the fume hood. Handling of hazardous material should be done by experienced experimenters only. In all cases, experimenters are responsible for following the CHESS safety guidelines and keeping experimental areas clean and neat, and for the prompt and complete removal of their solutions, affected glassware, and utensils at the end of their time at CHESS. |
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