Stephen Meisburger

Stephen Meisburger
Staff Scientist - XBio / FlexX
spm82@cornell.edu
Education/Experience
2022 – PresentStaff Scientist, CHESS, Cornell University
2018 – 2022Research Associate, Chemistry & Chemical Biology, Cornell University
2016 – 2018NIH Postdoctoral Fellow, Chemistry, Princeton University
2014 – 2016Postdoctoral Research Associate, Chemistry, Princeton University
2014Ph.D. in Applied Physics, Cornell University
2008 – 2011NIH Predoctoral Trainee in Molecular Biophysics, Cornell University
2007B.A. in Physics, Carleton College
Research Interests

Diffuse X-ray scattering from protein crystals, nucleic acid folding and interactions, enzyme allostery, and computational techniques for small-angle X-ray scattering

Selected Publications
  1. Meisburger, S. P.; Ando, N. Processing Macromolecular Diffuse Scattering Data. In Methods in Enzymology; Academic Press, 2023. https://doi.org/10.1016/bs.mie.2023.06.010.
  2. Pei, X.; Bhatt, N.; Wang, H.; Ando, N.; Meisburger, S. P. Introduction to Diffuse Scattering and Data Collection. In Methods in Enzymology; Academic Press, 2023. https://doi.org/10.1016/bs.mie.2023.07.007.
  3. Meisburger, S. P.; Case, D. A.; Ando, N. Robust Total X-Ray Scattering Workflow to Study Correlated Motion of Proteins in Crystals. Nat Commun 2023, 14 (1), 1228. https://doi.org/10.1038/s41467-023-36734-3.
  4. Meisburger, S. P.; Xu, D.; Ando, N. REGALS: A General Method to Deconvolve X-Ray Scattering Data from Evolving Mixtures. IUCrJ 2021, 8 (2), 225–237. https://doi.org/10.1107/S2052252521000555.
  5. Xu, D.; Meisburger, S. P.; Ando, N. Correlated Motions in Structural Biology. Biochemistry 2021, 60 (30), 2331–2340. https://doi.org/10.1021/acs.biochem.1c00420.
  6. Meisburger, S. P.; Case, D. A.; Ando, N. Diffuse X-Ray Scattering from Correlated Motions in a Protein Crystal. Nat Commun 2020, 11 (1), 1271. https://doi.org/10.1038/s41467-020-14933-6.
  7. Meisburger, S. P.; Ando, N. Correlated Motions from Crystallography beyond Diffraction. Acc. Chem. Res. 2017, 50 (3), 580–583. https://doi.org/10.1021/acs.accounts.6b00562.
  8. Plumridge, A.; Meisburger, S. P.; Andresen, K.; Pollack, L. The Impact of Base Stacking on the Conformations and Electrostatics of Single-Stranded DNA. Nucleic Acids Res. 2017. https://doi.org/10.1093/nar/gkx140.
  9. Plumridge, A.; Meisburger, S. P.; Pollack, L. Visualizing Single-Stranded Nucleic Acids in Solution. Nucl. Acids Res. 2016, gkw1297. https://doi.org/10.1093/nar/gkw1297.
  10. Meisburger, S. P.; Taylor, A. B.; Khan, C. A.; Zhang, S.; Fitzpatrick, P. F.; Ando, N. Domain Movements upon Activation of Phenylalanine Hydroxylase Characterized by Crystallography and Chromatography-Coupled Small-Angle X-Ray Scattering. Journal of the American Chemical Society 2016, 138 (20), 6506–6516. https://doi.org/10.1021/jacs.6b01563.
  11. Meisburger, S. P.; Pabit, S. A.; Pollack, L. Determining the Locations of Ions and Water around DNA from X-Ray Scattering Measurements. Biophysical Journal 2015, 108 (12), 2886–2895. https://doi.org/10.1016/j.bpj.2015.05.006.
  12. Meisburger, S. P.; Warkentin, M.; Chen, H.; Hopkins, J. B.; Gillilan, R. E.; Pollack, L.; Thorne, R. E. Breaking the Radiation Damage Limit with Cryo-SAXS. Biophys. J. 2013, 104 (1), 227–236. https://doi.org/10.1016/j.bpj.2012.11.3817.
  13. Yoo, T. Y.; Meisburger, S. P.; Hinshaw, J.; Pollack, L.; Haran, G.; Sosnick, T. R.; Plaxco, K. Small-Angle X-Ray Scattering and Single-Molecule FRET Spectroscopy Produce Highly Divergent Views of the Low-Denaturant Unfolded State. Journal of Molecular Biology 2012, 418 (3–4), 226–236. https://doi.org/10.1016/j.jmb.2012.01.016.
  14. Chen, H.; Meisburger, S. P.; Pabit, S. A.; Sutton, J. L.; Webb, W. W.; Pollack, L. Ionic Strength-Dependent Persistence Lengths of Single-Stranded RNA and DNA. PNAS 2012, 109 (3), 799–804. https://doi.org/10.1073/pnas.1119057109.
  15. Pabit, S. A.; Meisburger, S. P.; Li, L.; Blose, J. M.; Jones, C. D.; Pollack, L. Counting Ions around DNA with ASAXS. J Am Chem Soc 2010, 132 (46), 16334–16336. https://doi.org/10.1021/ja107259y.
Recorded Lectures

SBGrid weekly webinar series, “Deconvolving protein dynamics in solution & crystals with X-ray scattering”. April 6, 2021.

Relevant Links

Personal website

Complete publication list