Richard Gillilan

Education/Experience:

 2021             Fellow of the American Crystallographic Association

Research Interests:

Extreme biophysics, 3D proteomics, high pressure biology and deep life, small-angle X-ray solution scattering, biophysical instrumentation and separation science, x-ray optics, protein-protein interactions (simulation and experiment), aggregation, stability and phase separation, microbial fossils.

Article about my recent research (Physics Magazine): https://physics.aps.org/articles/v17/132

Recent Publications:

• Xia Y, Amann BT, Gillilan RE, Jiang Y, Sharma P, Sen S, Fleming KG, O’Brien EP, Fried SD (2026) Phosphoglycerate Kinase Can Adopt Topologically Misfolded Forms That Are More Stable Than Its Native State. J. Am. Chem. Soc. 148:4645–4659.
• Rinee KC, Patton ZE, Gillilan RE, Huang Q, Pingali SV, Heroux L, Xu AY (2025) Elucidating the porous structure of aluminum adjuvants via in-situ small-angle scattering technique. Vaccine 50:126813.
• Martinez Grundman JE, Schultz TD, Schlessman JL, Johnson EA, Gillilan RE, Lecomte JTJ (2025) Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N. Journal of Biological Chemistry 301:108223.
• Kimball WD, Lanzaro A, Hurd C, Jhaveri N, Huang J, Lewandowski J, Qian KK, Woldeyes MA, Majumdar R, Witek MA, et al. (2025) Growth of Clusters toward Liquid–Liquid Phase Separation of Monoclonal Antibodies as Characterized by Small-Angle X-ray Scattering and Molecular Dynamics Simulation. J. Phys. Chem. B 129:2856–2871.
• Gupta K, Sekulić N, Allu PK, Sapp N, Huang Q, Sarachan K, Christensen M, Lund R, Krueger S, Curtis JE, et al. (2025) Solution conformational differences between conventional and CENP-A nucleosomes are accentuated by reversible deformation under high pressure. Chromosome Research 33:11.
• Yuan G, Salipante PF, Hudson SD, Gillilan RE, Huang Q, Hatch HW, Shen VK, Grishaev AV, Pabit S, Upadhya R, et al. (2024) Flow Activation Energy of High-Concentration Monoclonal Antibody Solutions and Protein–Protein Interactions Influenced by NaCl and Sucrose. Mol. Pharmaceutics [Internet]. Available from: https://doi.org/10.1021/acs.molpharmaceut.4c00460
• Winnikoff JR, Milshteyn D, Vargas-Urbano SJ, Pedraza-Joya MA, Armando AM, Quehenberger O, Sodt A, Gillilan RE, Dennis EA, Lyman E, et al. (2024) Homeocurvature adaptation of phospholipids to pressure in deep-sea invertebrates. Science 384:1482–1488.
• Moran HM, Manriquez-Sandoval E, Sharma P, Fried SD, Gillilan RE (2024) Proteome-Wide Assessment of Protein Structural Perturbations under High Pressure. PRX Life 2:033011.
• Koduru T, Hantman N, Peters EV, Jaworek MW, Wang J, Zhang S, McCallum SA, Gillilan RE, Fossat MJ, Roumestand C, et al. (2024) A molten globule ensemble primes Arf1–GDP for the nucleotide switch. Proceedings of the National Academy of Sciences 121:e2413100121.
• Ko Y-H, Lokareddy RK, Doll SG, Yeggoni DP, Girdhar A, Mawn I, Klim JR, Rizvi NF, Meyers R, Gillilan RE, et al. (2024) Single Acetylation-mimetic Mutation in TDP-43 Nuclear Localization Signal Disrupts Importin α1/β Signaling. Journal of Molecular Biology 436:168751.
• Ko Y-H, Lokareddy RK, Doll SG, Yeggoni DP, Girdhar A, Mawn I, Klim JR, Rizvi NF, Meyers R, Gillilan RE, et al. (2024) Single Acetylation-mimetic Mutation in TDP-43 Nuclear Localization Signal Disrupts Importin α1/β Signaling. Journal of Molecular Biology 436:168751.
• Zhang M, Liu Y, Zuo X, Qian S, Pingali SV, Gillilan RE, Huang Q, Zhang D (2023) pH-Dependent Solution Micellar Structure of Amphoteric Polypeptoid Block Copolymers with Positionally Controlled Ionizable Sites. Biomacromolecules 24:3700–3715.
• Wang J, Koduru T, Harish B, McCallum SA, Larsen KP, Patel KS, Peters EV, Gillilan RE, Puglisi EV, Puglisi JD, et al. (2023) Pressure pushes tRNALys3 into excited conformational states. Proceedings of the National Academy of Sciences 120:e2215556120.
• Li Y, Li Q, Gillilan RE, Abbaspourrad A (2023) Reversible disassembly-reassembly of C-phycocyanin in pressurization-depressurization cycles of high hydrostatic pressure. International Journal of Biological Macromolecules 253:127623.
• Illava G, Gillilan R, Ando N (2023) Development of in-line anoxic small-angle X-ray scattering and structural characterization of an oxygen-sensing transcriptional regulator. J Biol Chem:105039.
• Ford RR, Gilbert PH, Gillilan R, Huang Q, Donnelly R, Qian KK, Allen DP, Wagner NJ, Liu Y (2023) Micelle Formation and Phase Separation of Poloxamer 188 and Preservative Molecules in Aqueous Solutions Studied by Small Angle X-ray Scattering. Journal of Pharmaceutical Sciences 112:731–739.
• Chowdhury AA, Manohar N, Lanzaro A, Kimball WD, Witek MA, Woldeyes MA, Majumdar R, Qian KK, Xu S, Gillilan RE, et al. (2023) Characterizing Protein–Protein Interactions and Viscosity of a Monoclonal Antibody from Low to High Concentration Using Small-Angle X-ray Scattering and Molecular Dynamics Simulations. Mol. Pharmaceutics [Internet]. Available from: https://doi.org/10.1021/acs.molpharmaceut.3c00484
• Biswas S, Hecht AL, Noble SA, Huang Q, Gillilan RE, Xu AY (2023) Understanding the Impacts of Molecular and Macromolecular Crowding Agents on Protein–Polymer Complex Coacervates. Biomacromolecules [Internet]. Available from: https://doi.org/10.1021/acs.biomac.3c00545

Full publication list on Google Scholar: https://scholar.google.com/citations?hl=en&user=nj2c0oEAAAAJ&view_op=list_works&sortby=pubdate 

Scopus: https://www.scopus.com/authid/detail.uri?authorId=6701426897

Recent Recorded Lectures: 

• Structural Proteomics Under Deep Ocean Pressure (10 min; 2024) https://youtu.be/P2Eb8SMF33M
• Introduction to small-angle biological X-ray solution scattering: https://www.youtube.com/watch?v=NDU4S8DyH3I
• Biological scattering under high pressure https://www.youtube.com/watch?v=KYVNtNydMsE

Youtube channel: https://www.youtube.com/user/rgill333

CV: CV_09_10_24.pdf