Data, processing, and other resources

Book

Data collected at CHESS can be retrieved via Globus or our Linux login node.

Connect to /nfs/chess/raw/current/id7a/ to download your directories.

MacCHESS is the home of the widely-used BioSAXS data analysis package RAW. The program is free, open-source, and runs on Mac, Windows, and Linux.
Download and learn more about BioXTAS RAW.
Read the Docs includes helpful tutorials and guides for data processing. 

Other useful processing software:

  1. ATSAS data analysis software - program suite for small-angle scattering data analysis
  2. Situs - for modeling and visualization of low resolution bead models
  3. ScatterBrain - SAXS data reduction program developed at the Australian Synchrotron
  4. Fitting SAXS data on disordered proteins - Sosnick lab (U. Chicago)
  5. CCP-SAS project
  6. Bilbo MD

MacCHESS staff can teach you how to create scattering profiles, evaluate data quality, and compute Rg on site. But, just like protein crystallography, there is a lot to learn … much more than you can absorb in one sitting. We strongly recommend that new users take a training course, if possible. Such 1-2 day courses are offered by a number of synchrotron sources. MacCHESS periodically offers an introductory workshop entitled "BioSAXS Essentials." To receive notification of the next scheduled course offering, please leave us your contact information.

Review articles

  1. Jacques, D. A. and J. Trewhella (2010). Small-angle scattering for structural biology — Expanding the frontier while avoiding the pitfalls. Protein Science 19(4): 642-657.
  2. Putnam, C. D., M. Hammel, et al. (2007). X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Quarterly Reviews of Biophysics, 40(3): 191-285.
  3. Mertens, H. D. T. and D. I. Svergun (2010). Structural characterization of proteins and complexes using small-angle X-ray solution scattering. Journal of Structural Biology, 172(1): 128-141.
  4. Svergun, D. I. and M. H. J. Koch (2003). Small-angle scattering studies of biological macromolecules in solution. Reports on Progress in Physics, 66(10): 1735-1782.

Classic Texts

  1. Feigin, L. A. and D. I. Svergun (1987). Structure Analysis by Small-Angle X-ray and Neutron Scattering, Plenum Press/Springer.
  2. Glatter, O. and Kratky (1982). Small-angle X-ray Scattering. London, Academic Press.
  3. Guinier, A. and G. Fourne (1955). Small-Angle Scattering of X-Rays. New York, John Wiley and Sons.

High-Pressure workshop slides