Macromolecular crystallography(MX), Standard cryocrystallography, Serial crystallography, High-Pressure cryocooling, MX experiments under pressure in diamond anvil cell (DAC)
Structure from a fluoroacetate dehalogenase crystal obtained at the FlexX beamline.
Description: |
Macromolecular crystallography (MX), including standard cryocrystallography, serial crystallography at room temperature, crystallography under high pressure in diamond anvil cell (MX-DAC). High-pressure cryocooling to reduce disorder in crystals. Development of new X-ray techniques for structural biology. |
Lead Scientists: |
Stephen Meisburger (lead); Zhongwu Wang |
Capabilities under development: |
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Anticipated availability of beamline capabilities: |
October 2019: Standard MX, including remote and mail-in modes. December 2019: Semi-routine serial crystallography, first tests of MX-DAC. |
Photon Energy Range: |
8-14 keV at 0.6% bandwidth |
Energy Resolution: |
0.6% bandwidth |
Spot size: |
9 µm (H) x 12 µm (V) with microfocusing CRL; 100 µm circular diameter with collimator |
This undulator-fed beamline spans an energy range of 8-14 keV. It features multilayer monochromator optics at 0.6% bandpass for high flux, capable of delivering 1012 ph/s into a 100 µm x 100 µm spot size. Microfocusing optics are available to give a beam size down to approximately 10 µm x 10 µm. FlexX currently houses a Eiger2 16M detector. The BAM-2 style automounter accepts Unipucks for fast sample changing. Remote data collection is available for routine experiments. We welcome users who wish to do non-standard experiments that require more time for setup and collection, such as serial crystallography, room temperature crystallography, crystallography at high pressure, diffuse scattering, and other experiments.