Macromolecular crystallography(MX), Standard cryocrystallography, Serial crystallography, High-Pressure cryocooling, MX experiments under pressure in diamond anvil cell (DAC)
Macromolecular crystallography (MX), including standard cryocrystallography, serial crystallography at room temperature, crystallography under high pressure in diamond anvil cell (MX-DAC).
High-pressure cryocooling to reduce disorder in crystals.
Development of new X-ray techniques for structural biology.
Anticipated availability of beamline capabilities:
October 2019: Standard MX, including remote and mail-in modes.
December 2019: Semi-routine serial crystallography, first tests of MX-DAC.
Photon Energy Range:
8-14 keV at 0.6% bandwidth
Energy Resolution:
0.6% bandwidth
Spot size:
9 µm (H) x 12 µm (V) with microfocusing CRL; 100 µm circular diameter with collimator
This undulator-fed beamline spans an energy range of 8-14 keV. It features multilayer monochromator optics at 0.6% bandpass for high flux, capable of delivering 1012 ph/s into a 100 µm x 100 µm spot size. Microfocusing optics are available to give a beam size down to approximately 10 µm x 10 µm. FlexX currently houses a Eiger2 16M detector. The BAM-2 style automounter accepts Unipucks for fast sample changing. Remote data collection is available for routine experiments. We welcome users who wish to do non-standard experiments that require more time for setup and collection, such as serial crystallography, room temperature crystallography, crystallography at high pressure, diffuse scattering, and other experiments.
High pressure is a tool that can be used to alter the equilibrium population of molecular conformations and associations, both in crystals and in solutions.