Richard Gillilan

Education/Experience:

2021 Fellow of the American Crystallographic Association

2000-present	Sr Staff Scientist, Cornell High Energy Synchrotron Source, Cornell University
1992-2000	Research Scientist, Visualization Specialist, Sr Tech Advisor, Cornell Theory Center
1990-1992	Postgraduate Research Chemist, University of California, San Diego
1988-1990	Postdoctoral Research Associate, Chemistry, Cornell University
1988	Ph.D. Chemistry, University of Pennsylvania
1983	B.A. Chemistry (honors), Mathematics, University of Maryland, Baltimore County

Research Interests:

High pressure biology, extreme biophysics, structural proteomics, deep life, small-angle X-ray solution scattering, biophysical instrumentation and separation science, x-ray optics, protein-protein interactions (simulation and experiment), aggregation, stability and phase separation, origin of life.

Recent Publications:

Li, Y., Li, Q., Gillilan, R. E. & Abbaspourrad, A. Reversible disassembly-reassembly of C-phycocyanin in pressurization-depressurization cycles of high hydrostatic pressure. International Journal of Biological Macromolecules 253, 127623 (2023).
Chowdhury, A. A. et al. Characterizing Protein–Protein Interactions and Viscosity of a Monoclonal Antibody from Low to High Concentration Using Small-Angle X-ray Scattering and Molecular Dynamics Simulations. Mol. Pharmaceutics (2023) doi:10.1021/acs.molpharmaceut.3c00484.
Biswas, S. et al. Understanding the Impacts of Molecular and Macromolecular Crowding Agents on Protein–Polymer Complex Coacervates. Biomacromolecules (2023) doi:10.1021/acs.biomac.3c00545.
Illava, G., Gillilan, R. & Ando, N. Development of in-line anoxic small-angle X-ray scattering and structural characterization of an oxygen-sensing transcriptional regulator. J Biol Chem 105039 (2023) doi:10.1016/j.jbc.2023.105039.
Peters, E. et al. Defining the role of the N-terminal helix in the Arf1 nucleotide switch transition using pressure perturbation. Biophysical Journal 122, 329a (2023).
Zhang, M. et al. pH-Dependent Solution Micellar Structure of Amphoteric Polypeptoid Block Copolymers with Positionally Controlled Ionizable Sites. Biomacromolecules acs.biomac.3c00407 (2023) doi:10.1021/acs.biomac.3c00407.
Wang, J. et al. Pressure pushes tRNALys3 into excited conformational states. Proceedings of the National Academy of Sciences 120, e2215556120 (2023).
Ford, R. R. et al. Micelle Formation and Phase Separation of Poloxamer 188 and Preservative Molecules in Aqueous Solutions Studied by Small Angle X-ray Scattering. Journal of Pharmaceutical Sciences 112, 731–739 (2023).
*Miller, R. C., Cummings, C., Huang, Q., Ando, N. & Gillilan, R. E. Inline small-angle X-ray scattering-coupled chromatography under extreme hydrostatic pressure. Protein Science 31, e4489 (2022). doi:10.1002/pro.4489
*Gillilan, R. E. High-pressure SAXS, deep life, and extreme biophysics. in Methods in Enzymology (Academic Press, 2022). doi:10.1016/bs.mie.2022.08.025.
Zhang, S., McCallum, S. A., Gillilan, R. E., Wang, J. & Royer, C. A. High Pressure CPMG and CEST Reveal That Cavity Position Dictates Distinct Dynamic Disorder in the PP32 Repeat Protein. J. Phys. Chem. B (2022) doi:10.1021/acs.jpcb.2c05498.
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland TE IV, Cowieson N, Crossett B, et al. (2022) A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking. Acta Crystallographica Section D [Internet] 78. Available from: https://doi.org/10.1107/S2059798322009184
Allsopp, R. et al. Antimicrobial Peptide Mechanism Studied by Scattering-Guided Molecular Dynamics Simulation. J. Phys. Chem. B (2022) doi:10.1021/acs.jpcb.2c03193.
Lokareddy, R. K. et al. Terminase Subunits from the Pseudomonas-Phage E217. Journal of Molecular Biology 434, 167799 (2022).
Florio, T. J. et al. Differential recognition of canonical NF-κB dimers by Importin α3. Nature Communications 13, 1207 (2022).
Doll SG, Meshkin H, Bryer AJ, Li F, Ko Y-H, et al. 2022. Recognition of the TDP-43 nuclear localization signal by importin α1/β. Cell Reports. 39(13):111007
Zovo, K. et al. Substitution of the Methionine Axial Ligand of the T1 Copper for the Fungal-like Phenylalanine Ligand (M298F) Causes Local Structural Perturbations that Lead to Thermal Instability and Reduced Catalytic Efficiency of the Small Laccase from Streptomyces coelicolor A3(2). ACS Omega (2022) doi:10.1021/acsomega.1c06668.